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Whole transcriptome analysis of postmortem human hippocampus dentate gyrus granlule cells

gse42546

Description

We investigated the transcriptome of dentate gyrus (DG) granule cells in postmortem hippocampus from 79 subjects with mental illness (schizophrenia, bipolar disorder, major depression) or non-psychiatric controls.

Overall Design

Material for RNA-seq analysis was harvested from tissue slides using laser capture microdissection (LCM) and aRNA amplification. Equimolar amounts of triplicate aRNA samples for each of the 79 subjects were then pooled for the preparation of sequencing libraries. Sequencing libraries were prepared using Applied Biosystem's Total RNA Sequencing Kit, following the directions for Whole Transcriptome Libraries, and analyzed with an Applied Biosystems SOLiD 4 high-throughput sequencer. We compared the performance of different normalization methods (length normalization vs. noise reductin scaling), and assembled evidence for dysruption of signaling by the micro RNA miR-182 in subjects with major depression and schizophrenia.

Histogram

Data and Resources

Raw Files [79]

Additional Info

Field Value
Source https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE42546
Type of Data

Expression profiling by high throughput sequencing

Technology

RNA Sequencing

GSE Submission Date 27/11/2012
GSE Authors Ruth,,Kohen; Adrian Dobra; Julia,H,Tracy; Eric Haugen
Dataset Last Updated November 29, 2019, 16:36 (UTC)
Dataset Created November 29, 2019, 13:01 (UTC)